| 引用本文: | 苏美英，周婷婷，周茂金.LC/MSn鉴定咖啡酸在大鼠体内的代谢产物[J].中国现代应用药学,2009,(6):501-505. |
| SU Meiying, ZHOU Tingting, ZHOU Maojin.The Characterization of the Metabolites of Caffeic Acid in Rats by LC/MSn[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(6):501-505. |
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| 摘要: |
| 目的研究咖啡酸（CA）在大鼠体内的代谢产物。方法大鼠灌胃（50 mg·kg-1）给予CA后采集0～4 h尿样，用电喷雾离子阱多级质谱法对CA在大鼠体内的代谢产物进行分析。结果大鼠灌胃给予CA后，在体内可测到2个原形药的甲基化代谢物、2个原形药的单葡萄糖醛酸结合物、1个原形药的双葡萄糖醛酸结合物、2个原形药的单硫酸结合物、2个甲基化物的葡萄糖醛酸结合物和2个甲基化物的硫酸结合物。结论CA在大鼠体内广泛代谢，其代谢物的结构有待于进一步分析后确证。 |
| 关键词: 咖啡酸 电喷雾离子阱多级质谱法 代谢物 |
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| The Characterization of the Metabolites of Caffeic Acid in Rats by LC/MSn |
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SU Meiying, ZHOU Tingting, ZHOU Maojin
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| Abstract: |
| OBJECTIVE To investigate the metabolites of caffeic acid (CA) in rats. METHODS After an oral administration of CA (50 mg·kg-1) to Wistar rats, 0-4 h rats urine was collected, and metabolites of CA in rats was determined by liquid chromatography tandem multi-stage mass spectrometry (LC/MSn). RESULTS The results revealed that two methylated derivatives (ferulic and isoferulic acid) of CA, two CA-glucuronides, two CA-sulfates, one CA-di-glucuronide, two methylated CA-glucuronides, two methylated CA-sulfates were found in rats urine. CONCLUSION CA was extensively metabolized in rats and structures of the metabolites will be further confirmed. |
| Key words: caffeic acid LC/MSn metabolites |
