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| 摘要: |
| 目的建立高效液相色谱法测定药材中氯化两面针碱含量的方法。方法采用色谱柱为SymmetryshielyTM RP18 柱（4.6 mm×250 mm, 5 μm），流动相为乙腈-0.04 mol·L-1醋酸钠溶液（用醋酸调节pH为6.5），检测波长为270 nm。结果 氯化两面针在0.25～5.0 μg内线性关系良好。平均回收率为99.6%，RSD为1.49% 。结论该方法简单准确，重复性好，可用于两面针药材的质量控制。 |
| 关键词: 两面针 氯化两面针碱 高效液相色谱法 |
| DOI： |
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| Determination of Nitidine Chloride in Zanthoxylum nitidum (Roxb．) DC．by HPLC |
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CHEN Qiuhong
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of nitidine chloride in Zanthoxylum nitidum (Roxb.) DC. METHODS The method contained a SymmetryshielyTM RP18 (4.6 mm×250 mm, 5 μm) as column, with acetonitrile-0.04 mol·L-1 NaAc（adjust pH to 6.5 with Hac）as mobile phase, UV detection wavelength at 270nm. RESULTS The calibration curve of nitidine chloride was linear between 0.25-5.0 μg.The average recovery was 99.6%, RSD=1.49%. CONCLUTION This method was proved to be simple, rapid, accurate and can be used for the quality control of Zanthoxylum nitidum（Roxb.）DC. |
| Key words: Zanthoxylum nitidum (Roxb.) DC nitidine chloride HPLC |
