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| 摘要: |
| 目的建立同时测定玄麦甘桔颗粒中甘草苷和甘草酸含量的HPLC。方法采用LiChrospher C18(250 mm×4.6 mm,5 μm)色谱柱，以乙腈-1.2%甲酸溶液为流动相，梯度洗脱，检测波长254 nm。结果甘草酸和甘草苷在0.005～0.5 mg·mL-1内呈良好的线性关系。甘草苷的回归方程为Y=5 735.789 7X-2.702 7,r=0.999 9，平均回收率为99.7%，RSD为1.72%（n=9），甘草酸的回归方程为Y=6 968.182 6X-3.448 4（r=0.999 8），平均回收率为100.8%，RSD为1.31%（n=9）。结论方法操作简便，结果可靠，重复性好，可作为同时测定玄麦甘桔颗粒中甘草苷与甘草酸含量的方法。 |
| 关键词: 高效液相色谱法 玄麦甘桔颗粒 甘草苷 甘草酸 |
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| Determination of Liquiritigenin and Glycyrrhizic Acid in Xuanmaiganjie Granule by HPLC |
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ZHANG Ping1,GUO Yadong2，MA Yinhai3*，XIONG Chunmei2
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of liquiritigenin and glycyrrhizic acid in Xuanmaiganjie granule.METHODS A LiChrospher C18column (250 mm×4.6 mm,5 μm) was used，the mobile phase was acetonitrile-water（1.2% methanoic acid） for gradient elution. The detection wavelength was set at 254 nm.RESULTS The linear range was 0.005-0.5 mg·mL-1.The regression equation of liquiritigenin was Y=5 735.789 7X-2.702 7(r=0.999 9)，and the average recovery was 99.7%（RSD=1.72%，n=9）.The regression equation of glycyrrhizic acid was Y=6 968.182 6X-3.448 4（r=0.999 8），and the average recovery was 100.8%（RSD=1.31%，n=9）.CONCLUSION The method is convenient and accurate. It can be used to determine the contents of liquiritigenin and glycyrrhizic acid in Xuanmaiganjie granule. |
| Key words: HPLC Xuanmaiganjie granule liquiritigenin glycyrrhizic acid |
