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| 摘要: |
| 目的建立乳疾宁胶囊中丹酚酸B的含量测定方法。方法色谱柱：Agilent ZORBAX Eclispse XDB-C18柱（4.6 mm×150 mm，5 μm），流动相：甲醇-乙腈-甲酸-水（28∶8∶1∶63），检测波长：286 nm。结果丹酚酸B在0.9～2.7 μg内呈良好线性关系，r=0.999 7，加样回收率平均为100.1%（n＝9，RSD＝2.2%）。结论本法灵敏度高、操作简便、结果准确，可用于乳疾宁胶囊中丹酚酸B的含量测定。 |
| 关键词: 乳疾宁胶囊 丹酚酸B 高效液相色谱法 含量测定 |
| DOI： |
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| Determination of Salvianolic Acid B in Rujining Capsule by HPLC |
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DUAN Xiaoying
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| Abstract: |
| OBJECTIVE To establish the method for determining the concentration of salvianolic acid B in Rujining capsule by HPLC. METHODS Using Agilent ZORBAX Eclispse XDB-C18(4.6 mm×150 mm,5 μm) as chromatographic column,methanol-acetonitrile-formic acid-water（28∶8∶1∶63）as mobile phase.The detection wavelength was 286 nm.RESULTS The standard curves of salvianolic acid B were linear in range of 0.9-2.7 μg,r=0.999 7.The average recovery of salvianolic acid B were 100.1%（n＝9，RSD＝2.2%）. CONCLUSION This method is sensitive,quick and accurate for the determination of salvianolic acid B in Rujining capsule. |
| Key words: Rujining capsule salvianolic acid B HPLC determination |
