| 引用本文: | 汤浩,闵光涛,李玉民,蒋生祥.HPLC同时测定大鼠血浆中克泻灵片的生物碱[J].中国现代应用药学,2009,(2):158-161. |
| TANG Hao1,2, MIN Guangtao3, LI Yumin3,JIANG Shengxiang1*.Simultaneous Determination of Alkaloids by HPLC in Rat Plasma after Kexieling Tablets Administration[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(2):158-161. |
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| 摘要: |
| 目的 建立克泻灵片中生物碱成分的大鼠血浆HPLC同时测定方法。方法 灌胃克泻灵片，以乙酰苯胺为内标，RP-HPLC-UV法在220 nm波长处进行血药浓度测定。结果 槐定碱和苦参碱在大鼠血浆中定量准确，最低检测限分别为350 ng·mL-1和100 ng·mL-1，回收率大于98%。结论 该方法简便、准确、重复性好，可为克泻灵片的体内药动学研究提供方法学参考。 |
| 关键词: 槐定碱 苦参碱 高效液相色谱法 大鼠血浆 克泻灵片 |
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| Simultaneous Determination of Alkaloids by HPLC in Rat Plasma after Kexieling Tablets Administration |
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TANG Hao1,2, MIN Guangtao3, LI Yumin3,JIANG Shengxiang1*
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| Abstract: |
| OBJECTIVE To establish an HPLC method for simultaneously determining the alkaloids of Kexieling tablets in rat plasma. METHODS The plasma samples were determined at 220 nm wavelength by reversed-phase HPLC and ultraviolet detection following oral administration of Kexieling tablets. RESULTS Sophoridine and matrine with 350 and 100 ng·mL-1of limits of quantitation in plasma, respectively, can be accurately quantitated, and the recovery of both analytes was greater than 98%.CONCLUSION The method is simple,accurate and can be referenced for pharmacokinetic study of Kexieling tablets. |
| Key words: sophoridine matrine HPLC rat plasma Kexieling tablets |
