| 引用本文: | 李万红,徐靖宏,陈虹,林军.RP-HPLC测定杜仲叶活性成分的含量[J].中国现代应用药学,2009,(2):140-142. |
| LI Wanhong1，XU Jinghong2*，CHEN Hong2，LIN Jun2.Determination of Active Constitutents in Folium Eucommiae by RP-HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(2):140-142. |
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| 摘要: |
| 目的建立同时测定杜仲叶中活性成分绿原酸、京尼平苷含量的反相高效液相色谱法。方法采用YMC-pack ODS-A色谱柱（250 mm×4.6 mm，5 μm），以甲醇-水-冰醋酸（20∶80∶1.5）为流动相，检测波长237 nm，柱温30 ℃，流速1.0 mL·min-1。结果绿原酸在0.041 6～0.624 0 μg内，有较好的线性关系(r=0.999 2)，绿原酸的加样回收率为102.92%，RSD为1.49%；京尼平苷在0.081 6～1.224 0 μg内，有较好的线性关系(r=0.998 9)，京尼平苷的加样回收率为102.35%，RSD为1.20%。杜仲叶中绿原酸含量为2.801 mg·g-1、京尼平苷含量为0.273 mg·g-1。结论所建立的方法简便、准确、可靠，可作为杜仲叶的质量控制方法。 |
| 关键词: 反相高效液相色谱法 杜仲叶 含量测定 绿原酸 京尼平苷 |
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| Determination of Active Constitutents in Folium Eucommiae by RP-HPLC |
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LI Wanhong1，XU Jinghong2*，CHEN Hong2，LIN Jun2
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| Abstract: |
| OBJECTIVE To establish a new reversed phase high-performance liquid chromatography (RP-HPLC) method for the determination of chlorogenic acid and genipoide, the active constitutents in folium eucommiae simultaneously. METHODS YMC-pack ODS-A column (250 mm×4. 6 mm, 5 μm) was used and the mobile phase was methanol-water-acetic acid (20∶80∶1.5) at the flow rate of 1.0 mL·min-1. The detection wavelength was 237 nm and the temperature of column was 30 ℃. RESULTS The linear range of chlorogenic acid and genipoide were 0.041 6-0.624 0 μg(r=0.999 2) and 0.081 6-1.224 0 μg(r=0.998 9). The average recoveries were 102.92% and 102.35%,respectively. The RSD were 1.49% and 1.20%. The content of chlorogenic acid and genipoide in folium eucommiae were 2.801 mg·g-1 and 0.273 mg·g-1. CONCLUSION The method is simple, accurate and reliability, with good repeatability, and can be used for the quality control of folium eucommiae. |
| Key words: RP-HPLC folium eucommiae content determination chlorogenic acid genipoide |
