| 引用本文: | 雷丹青;刘绵林;周先丽.发色底物法测定注射用降纤酶中类凝血酶的含量[J].中国现代应用药学,2005,(6):482-483. |
| LEI Dan-qing,LIU Mian-lin,ZHOU Xian-li.Determination of thrombin-like in defibrase for injection by chromogenic substrate[J].Chin J Mod Appl Pharm(中国现代应用药学),2005,(6):482-483. |
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| 摘要: |
| 目的建立发色底物法测定注射用降纤酶类凝血酶的方法。方法以Be-Phe-Val-Arg-PNA为发色底物,检测波长405nm。结果降纤酶在2～10单位/mL范围内呈良好的线性关系,r=0.9993(n=5)。结论本法简便,准确,专属性强,适用于产品的质量控制。 |
| 关键词: 降纤酶 类凝血酶 发色底物 含量测定 |
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| 基金项目:广西壮族自治区自然科学基金资助项目(0342002-4) |
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| Determination of thrombin-like in defibrase for injection by chromogenic substrate |
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LEI Dan-qing,LIU Mian-lin,ZHOU Xian-li
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| Abstract: |
| OBJECTIVE A chromogenic substrate method was developed for the determination of thrombin-like in defibrase for injection.METHODS Chromogenic substrate was Be-Phe-Val-Arg-PNA.The detection was made at 405nm. RESULTS The calibration curve of thrombin-like h |
| Key words: defibrase thrombin-like chromogenic substrate determination |
