引用本文: | 汪凤梅;姚彤炜;吴越1;曾苏2.HPLC测定人肝转基因细胞中的盐酸维拉帕米[J].中国现代应用药学,2001,(5):379-381. |
| Wang Feng Mei ,Yao Tong Wei,Wu Yue 1, Zeng Su 2.Determination of Verapamil Metabolism in cDNA-expressed cells by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2001,(5):379-381. |
|
|
摘要: |
|
关键词: 维拉帕米 反相高效液相色谱 细胞色素P450转基因细胞 孵育 |
DOI: |
分类号: |
基金项目:国家自然科学基金 ( No 39370805) ;Tel:(0571)87217203;E-mail:zengsu@yahoo.com |
|
Determination of Verapamil Metabolism in cDNA-expressed cells by HPLC |
Wang Feng Mei ,Yao Tong Wei,Wu Yue 1, Zeng Su 2
|
Abstract: |
OBJECTIVE:To establish a RP HPLC method for the determination of verapamil after metabolism by cytochrome P450 cDNA transgenic cell homogenate. METHOD:The determination was performed on a C18 reversed phase column with a mobile phase composed of methano |
Key words: Verapamil, RP HPLC ,Cytochrome p450 cDNA transgenic cell, Incubation |