| 引用本文: | 吴越,颜冬梅,屠凌岚,郑晓亮.三溴偶氮氯膦褪色分光光度法测定顺铂注射液的含量[J].中国现代应用药学,2011,28(6):558-560. |
| WU Yue,YAN Dongmei,TU Linglan,ZHENG Xiaoliang.Catalytic Spectrophotometric Determination of Trace Cisplatin Injection by Tribromochlorophosphonazo[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(6):558-560. |
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| 摘要: |
| 目的 建立催化动力学分光光度法测定常用抗肿瘤药顺铂注射液含量的方法。方法 在硫酸介质中，进行顺铂催化溴酸钾氧化三溴偶氮氯膦的褪色反应，褪色程度与顺铂含量呈线性关系，利用催化动力学分光光度法进行测定。结果 顺铂-三溴偶氮氯膦-溴酸钾反应液的最大吸收峰在530 nm，3~24 ng·mL-1内线性关系良好(r=0.999 1)，平均回收率为99.3%，RSD为1.24%(n=3)。结论 本方法灵敏度高、操作简便快速，可用于顺铂注射剂的含量测定。 |
| 关键词: 顺铂注射液 三溴偶氮氯膦 催化分光光度法 含量测定 |
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| Catalytic Spectrophotometric Determination of Trace Cisplatin Injection by Tribromochlorophosphonazo |
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WU Yue1, YAN Dongmei2, TU Linglan2, ZHENG Xiaoliang2
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1.Zhejiang Provincial People’s Hospital, Hangzhou 310014, China;2.Zhejiang Academy of Medical Sciences, Hangzhou 310013, China
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| Abstract: |
| OBJECTIVE To establish a catalytic spectrophotometric method for determination of trace cisplatin injection. METHODS A catalytic spectrophotometric method based on the catalytic effect of platinum on the oxidation of tribromochlorophosphonazo [3-(2, 4, 6-tribromophenylazo)-6-(4-chloro-2-phosponophenylazo)-4,5-dihydoxynaphthalene-2,7- disufonic acid] with KBrO3 in acidic medium had been proposed for the determination of trace cisplatin. The fading reaction was performed directly in an aqueous solution and the optimal conditions were given. RESULTS The calibration curves were linear over the concentration range of 3-24 ng·mL-1 and the average recoveries of cisplatin were 99.3%, the RSD was 1.24%(n=3). CONCLUSION The catalytic spectrophotometric method is simple, rapid and sensitive, and could be used for the determination of cisplatin injection. |
| Key words: cisplatin injection tribromochlorophosphonazo catalytic spectrophotometer content determination |
