| 引用本文: | 马舒伟，陈旅翼，何盛江，梁军，刘红霞，张现涛.银杏内酯K对脑缺血的保护作用[J].中国现代应用药学,2011,28(10):877-880. |
| MA Shuwei, CHEN Lüyi, HE Shengjiang, LIANG Jun, LIU Hongxia, ZHANG Xiantao.Protective Effect of Ginkgolide K on Cerebral Ischemia[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(10):877-880. |
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| 摘要: |
| 目的 研究银杏内酯K对脑缺血的保护作用。方法 采用小鼠常压耐缺氧实验，测定小鼠存活时间；小鼠双侧颈总动脉结扎引起急性不完全脑缺血模型，测定小鼠喘气时间、脑乳酸含量和碱性磷酸酶活性，观察组织形态学、脑指数和脑含水量的变化。结果 银杏内酯K能剂量依赖性地延长小鼠缺氧缺血的存活时间；延长喘气时间，减少乳酸含量，降低碱性磷酸酶活性，减小脑指数和脑含水量。病理学组织检查显示，银杏内酯K能改善脑缺血再灌注造成的小鼠神经细胞的损伤，减少组织坏死。结论 银杏内酯K对脑缺血有保护作用。 |
| 关键词: 银杏内酯K 脑缺血 缺氧 乳酸 碱性磷酸酶 脑含水量 |
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| 基金项目:国家自然科学基金(81001384)；广州市科技计划项目(2010GK-C041) |
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| Protective Effect of Ginkgolide K on Cerebral Ischemia |
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MA Shuwei, CHEN Lüyi, HE Shengjiang, LIANG Jun, LIU Hongxia, ZHANG Xiantao1,2,3
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1.Department of Pharmacy Engineering, Institute of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;2.Institute of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210009, China;3.Traditional Chinese Medicine Institue of Guangdong Province, Guangzhou 510009, China
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| Abstract: |
| OBJECTIVE To study the protective effect of ginkgolide K on cerebral ischemia. METHODS Anoxia was produced by close normobaric hypoxia; the survival time of mice was determined; acute incomplete cerebral ischemia was induced by ligaturing bilateral carotid arterise; breath time, brain lactic acid content and alkaline phosphatase activities, brain index and brain water content were detected, morphologic change was observed. RESULTS Ginkgolide K prolonged the survival time of hypoxic-ischemic mice in dose-dependent manner. In acute incomplete cerebral ischemia mice, ginkgolide K extended breathing time, reduced lactic acid content, decreased alkaline phosphatase activity, lessened brain index and brain water content. Moreover, pathological examination showed that ginkgolide K ameliorated the cerebral ischemia-reperfusion injury in mice nerve cells and reduced tissue necrosis. CONCLUSION Ginkgolide K has a protective effect on ischemia brain damage. |
| Key words: ginkgolide K cerebral anoxia cerebral ischemia lactic acid alkaline phosphatase brain water content |
