| 引用本文: | 石杰，陈安进，姜新道，张新惠，王本坚.D-聚甘酯抗凝活性与凝血因子Ⅹ/Ⅱ灭活度比值相关性研究[J].中国现代应用药学,2011,28(8):694-696. |
| SHI Jie, CHEN Anjin, JIANG Xindao, ZHANG Xinhui, WANG Benjian.Relationship Between Anticoagulation and Deactivation Ratio on Ⅹ/Ⅱ Factor of D-polymannuronicate[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(8):694-696. |
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| 摘要: |
| 目的 通过探索D-聚甘酯抗凝活性与Ⅹ/Ⅱ因子灭活度比值的相关性，推断其可能的抗凝抗栓机制，为临床更加合理应用该药提供参考。方法 设4个不同D-聚甘酯剂量组以及空白、肝素钠2个对照组，每组均测定凝血酶原时间(PT)，凝血活酶时间(APTT)以及Ⅱ因子、Ⅹ因子的活性，并计算Ⅹ/Ⅱ的灭活度比值。结果 在50~150 μg·mL-1内，随着D-聚甘酯浓度的增加，其抗凝活性增强，Ⅹ/Ⅱ因子灭活度比值升高。结论 D-聚甘酯在发挥抗凝活性时，可能的机制是以灭活凝血酶活性为主，而不是以抑制凝血酶原产生为主。 |
| 关键词: D-聚甘酯 抗凝活性 灭活度比值 相关性 |
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| 基金项目:中国海洋大学海洋药物教育部重点实验室开放基金资助项目[KLMD(OUC)200808] |
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| Relationship Between Anticoagulation and Deactivation Ratio on Ⅹ/Ⅱ Factor of D-polymannuronicate |
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SHI Jie, CHEN Anjin, JIANG Xindao, ZHANG Xinhui, WANG Benjian1,2
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1.Qingdao Municipal Hospital, Qingdao 266071, China;2.Ocean University of China, Qingdao 266003, China
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| Abstract: |
| OBJECTIVE To investigate relationship between anticoagulation and deactivation ratio on Ⅹ/Ⅱ factor of D-polymannuronicate and estimate the anticoagulation mechanism. METHODS Plasma sample was divided into 6 groups: four D-polymannuronicate groups in different doses, one blank group and one heparin sodium group. APTT, PT and the activity of factor Ⅱ, Ⅹ in each group were determined, then the deactivation ratio on Ⅹ/Ⅱ factor was calculated. RESULTS The anticoagulation as well as the deactivation ratio on Ⅹ/Ⅱ factor was enhanced with the increased concentration of D-polymannuronicate of 50-150 μg·mL-1. CONCLUSION The possible anticoagulation mechanism of D-polymannuronicate is deactivating the activity of thrombin rather than inhibiting thrombinogen. |
| Key words: D-polymannuronicate anticoagulation deactivation ratio relationship |
