| 引用本文: | 邢楠楠,赵雨,刘宏,刘海龙,王涵.人参出苗期几种水解酶的活力变化[J].中国现代应用药学,2011,28(5):429-431. |
| XING Nannan,ZHAO Yu,LIU Hong,LIU Hailong,WANG Han.Changes of Several Hydrolase Activities in the Period of Emergence in Ginseng Radix et Rhizoma[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(5):429-431. |
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| 摘要: |
| 目的 研究五年生人参几种水解酶在出苗期的活力变化。方法 采用中性磷酸缓冲溶液提取粗酶液。应用紫外分光光度法分别测定淀粉酶(AMY)、酯酶(Est)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(ALP)和植酸酶的活力。结果 人参根部Est、ACP和ALP活力都是先增加后下降再增加；幼苗 Est活力在出苗期前7 d增加较快之后无明显变化，ACP活力在出苗期第10天出现一个峰值，ALP活力始终保持上升趋势；人参根部AMY和植酸酶活力变化趋势相似，在出苗期第4至13天活力迅速增加，之后下降；而在幼苗中两种酶的变化趋势完全相反。结论 在出苗期，人参5种水解酶活力变化曲线有所差异，这可作为人参出苗期幼苗形态不同阶段的生理指标。 |
| 关键词: 人参 出苗期 水解酶 |
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| 基金项目:国家自然科学基金资助项目(30873371)；国家科技支撑计划资助项目(2007BAI38B02) |
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| Changes of Several Hydrolase Activities in the Period of Emergence in Ginseng Radix et Rhizoma |
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XING Nannan,ZHAO Yu,LIU Hong,LIU Hailong,WANG Han
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| Abstract: |
| OBJECTIVE To study changes of several hydrolase activities in the period of emergence in five years of Ginseng Radix et Rhizoma. METHODS Neutral phosphate buffer was selected to extract crude extract. UV was applied to determine the activities of amylase (AMY), esterase (Est), acid phosphatase (ACP), alkaline phosphatase (ALP), and phytase. RESULTS The trend of Est, ACP, and ALP activities increased first and then decreased to increase. Est activity increased rapidly in the first 7 days, after which there was no significant change in the period of emergence. ACP activity reached a peak on the 10th day of the period of emergence. ALP activity remained steady upward trend. The trend of AMY was similar to phytase in ginseng root. In the first 4-13 days, activity increased rapidly, then decreased. The trend of two enzymes activities was completely opposite in seedlings. CONCLUSION Five hydrolase activity curves are different, which can be used as a physiological index of ginseng seedling morphogenesis at different stages of period of emergence. |
| Key words: Ginseng Radix et Rhizoma period of emergence hydrolase |
