| 引用本文: | 卢平华,钱陈钦,黄晓琼.HPLC测定番石榴叶中金丝桃苷的含量[J].中国现代应用药学,2011,28(6):553-555. |
| LU Pinghua,QIAN Chenqin,HUANG Xiaoqiong.Determination of Hyperin in Psidium Guajava Leaf by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(6):553-555. |
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| 摘要: |
| 目的 建立番石榴叶中金丝桃苷的HPLC含量测定方法。方法 采用Dikma Kromasil C18色谱柱(4.6 mm×250 mm，5 mm)，以甲醇-0.1%磷酸溶液(45∶55)为流动相，流速为1.0 mL·min-1，检测波长为254 nm。结果 金丝桃苷在0.020 48~ 0.204 8 μg内呈良好的线性关系(r=0.999 9)，平均回收率为98.4%，RSD为1.5%。结论 本方法简单、准确、重复性好、专属性强，可用于番石榴叶药材及制剂中金丝桃苷的含量测定。 |
| 关键词: 高效液相色谱法 番石榴叶 金丝桃苷 |
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| Determination of Hyperin in Psidium Guajava Leaf by HPLC |
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LU Pinghua, QIAN Chenqin, HUANG Xiaoqiong
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Guangzhou Hanses Pharmaceutical R & D Co., Ltd., Guangzhou 510330, China
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| Abstract: |
| OBJECTIVE To establish a determination method for hyperin in Psidium guajava leaf by HPLC. METHODS HPLC was performed on a Dikma Kromasil C18 column(4.6 mm×250 mm, 5 mm) with methanol-0.1% phosphoric acid solution(45∶55) as the mobile phase at a flow rate of 1.0 mL·min-1, and the detection wavelength was 254 nm. RESULTS It showed a good linearity at the range of 0.020 48~0.204 8 mg(r=0.999 9), and the average recovery of hyperin was 98.4%(RSD=1.5%). CONCLUSION The method is sensitive, accurate, reproducible, specific and could be used to determine hyperin in Psidium guajava leaf. |
| Key words: HPLC Psidium guajava leaf hyperin |
