| 引用本文: | 华俊彦,朱雅艳,田伟强,赵静.HPLC测定人血浆中头孢噻利的血药浓度[J].中国现代应用药学,2011,28(6):548-550. |
| HUA Junyan,ZHU Yayan,TIAN Weiqiang,ZHAO Jing.Determination of Cefoselis in Human Plasma by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(6):548-550. |
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| 摘要: |
| 目的 建立高效液相色谱法测定人血浆中头孢噻利血药浓度的方法。方法 以甲硝唑为内标，色谱柱为ZORBAX Extend-C18(4.6 mm×250 mm，5 μm)，以乙腈-pH 6.5磷酸盐缓冲液(5.5∶94.5)为流动相，流速为1.0 mL·min-1，检测波长为254 nm，进样量为20 μL，柱温为25 ℃。结果 头孢噻利的血药浓度在1~200 mg·L-1内线性良好(r=0.999 9)，定量限为1 mg·L-1；平均方法回收率为99.8%(RSD=1.53%)；日内、日间RSD均<3％。结论 本方法简便、灵敏、快速、准确，可用于头孢噻利的临床药动学研究和生物利用度研究。 |
| 关键词: 头孢噻利 高效液相色谱法 血药浓度 |
| DOI： |
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| 基金项目:浙江省医学会基金项目(2009ZYC53) |
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| Determination of Cefoselis in Human Plasma by HPLC |
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HUA Junyan, ZHU Yayan, TIAN Weiqiang, ZHAO Jing
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Department of Pharmacy, Lishui Central Hospital, Lishui 323000, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of cefoselis in human plasma. METHODS With metronidazole as the internal standard, the separation of sample was carried out on a ZORBAX Extend C18 column (4.6 mm×250 mm, 5 μm) with the mobile phase consisted of phosphate buffer (pH 6.5)-acetonitrile (94.5∶5.5) at a flow rate of 1.0 mL·min-1. The detection wavelength was 254 nm, injected sample volume was 20 μL, and the column temperature was set at 25 ℃. RESULTS The linear range of cefoselis was 1-200 mg·L-1 (r=0.999 9) with the lower limit of quantization of 1 mg·L-1. The average methodological recovery was 99.8%(RSD=1.53%). The inter-day RSD and intra-day RSD were all less than 3%. CONCLUSION The method is simple, sensitive, rapid, and accurate, and can be used for clinical pharmacokinetic and bioavailability study of cefoselis. |
| Key words: cefoselis HPLC plasma drug concentration |
