| 摘要: |
| 目的 建立混合糖电解质注射液中果糖、葡萄糖和木糖醇的含量测定方法。方法 色谱柱：Waters Sugar Pak I(300 mm×6.5 mm)；流动相：水；流速：0.5 mL·min-1；示差折光检测器。结果 果糖、葡萄糖和木糖醇峰分离度良好；果糖的线性范围为20~100 μg(r=0.999 9)；平均回收率为99.53%，RSD=0.81%(n=9)；葡萄糖的线性范围为40~200 μg(r=0.999 7)；平均回收率为99.75%，RSD=0.47%(n=9)；木糖醇的线性范围为10~50 μg(r=0.999 7)；平均回收率为99.78%，RSD=1.02%(n=9)。结论 本方法简便、准确，可用于混合糖电解质注射液中果糖、葡萄糖和木糖醇的含量测定。 |
| 关键词: 混合糖电解质注射液 果糖 葡萄糖 木糖醇 高效液相色谱法 |
| DOI： |
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| Determination of Fructose, Glucose and Xylitolum in Carbohydrate and Electrolyte Injection by HPLC |
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ZHANG Xiaoli
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Changxing People’s Hospital, Huzhou 313100, China
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| Abstract: |
| OBJECTIVE To establish a method for determination of fructose, glucose and xylitolum in carbohydrate and electrolyte injection by HPLC. METHODS A Waters Sugar Pak I column (300 mm×6.5 mm) was used with the mobile phase of water. Flow rate was 0.5 mL·min-1. The tests were carried out by refractive index detector. RESULTS The resolution among fructose, glucose and xylitolum were good. The linear range of fructose was 20-100 μg(r=0.999 9), with average recovery of 99.53%, RSD=0.81%(n=9). The linear range of glucose was 40-200 μg (r=0.999 7), with average recovery of 99.75%, RSD=0.47%(n=9). The linear range of xylitolum was 10-50 μg (r=0.999 7), with average recovery of 99.78%, RSD=1.02%(n=9). CONCLUSION The established method is simple, sensitive and reproducible, and suitable for the quality control of multiple electrolytes and invert sugar injection. |
| Key words: carbohydrate and electrolyte injection fructose glucose xylitolum HPLC |
