| 引用本文: | 王剑霞,黄光亮,陈禧翎,段敏丽,罗昕.HPLC同时测定大豆磷脂中磷脂酰胆碱和溶血磷脂酰胆碱含量[J].中国现代应用药学,2011,28(6):564-566. |
| WANG Jianxia,HUANG Guangliang,CHEN Xiling,Duan Minli,LUO Xin.Determination of Phosphatidylcholine and Lysophosphatidylcholine in Soybean Phospholipids by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(6):564-566. |
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| 摘要: |
| 目的 建立同时测定大豆磷脂中磷脂酰胆碱(PC)和溶血磷脂酰胆碱(LPC)含量的方法。方法 采用高效液相色谱法，色谱柱为Phenomenex silica柱(4.6 mm×150 mm，5 μm)，流动相为正己烷-异丙醇-水(5∶80∶20)，流速：0.7 mL·min-1，检测波长：206 nm，柱温：25 ℃。结果 PC在0.4~2.4 mg·mL-1内呈良好的线性关系，平均回收率为99.4%，RSD为0.40%(n=6)；LPC在10~60 μg·mL-1内呈良好的线性关系，平均回收率为98.0%，RSD为1.65%(n=6)。结论 本试验方法简便、重复性好、专属性强，可作为大豆磷脂中PC，LPC的检测方法。 |
| 关键词: 大豆磷脂 磷脂酰胆碱 溶血磷脂酰胆碱 含量测定 |
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| Determination of Phosphatidylcholine and Lysophosphatidylcholine in Soybean Phospholipids by HPLC |
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WANG Jianxia1, HUANG Guangliang1, CHEN Xiling1, Duan Minli1, LUO Xin2
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1.Guangzhou Baiyunshan Mingxing Pharmaceutical Co., Ltd., Guangzhou 510250, China;2.School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for simultaneously determining phosphatidylcholine (PC), lysophosphatidylcholine (LPC) in soybean phospholipids. METHODS The HPLC system consisted of a Phenomenex silica column(4.6 mm×150 mm, 5 μm), a mobile phase of n-hexane-isopropanol-water(5∶80∶20). The flow rate was 0.7 mL·min-1. Column temperature was set at 25 ℃ and the wavelength of detector was set at 206 nm. RESULTS The calibration curve was linear in the range of 0.4-2.4 mg·mL-1 for PC and 10-60 μg·mL-1 for LPC, respectively. The average recovery for PC was 99.4% and RSD was 0.40%(n=6). The average recovery for LPC was 98.0% and RSD was 1.65%(n=6). CONCLUSION The method is simple, rapid and specially. It can be used for the determination of PC and LPC in soybean phospholipids. |
| Key words: soybean phospholipids phosphatidylcholine lysophosphatidylcholine determination |
