| 引用本文: | 王晓平,段丽菊,黄翔,岑业文,李桂芬.五指毛桃水提液对60Coγ射线致小鼠骨髓细胞DNA损伤的防护作用[J].中国现代应用药学,2011,28(4):284-287. |
| WANG Xiaoping,DUAN Liju,HUANG Xiang,CEN Yewen,LI Guifen.Protective Role of Aqueous Extract from Fici Hirtae Radix for DNA Damage of Bone Marrow Cells by 60Coγ-Ray in Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(4):284-287. |
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| 五指毛桃水提液对60Coγ射线致小鼠骨髓细胞DNA损伤的防护作用 |
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王晓平1,2, 段丽菊3, 黄翔1,2, 岑业文1,2, 李桂芬1,2
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1.广西玉林师范学院，a.化学与生物系;2.b.体育系，广西 玉林 537000;3.郑州大学公共卫生学院, 郑州 450001
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| 摘要: |
| 目的 探讨五指毛桃水提液对60Coγ射线所致的小鼠骨髓细胞DNA损伤的防护作用。方法 40只SPF级♂小鼠随机分为5组：阴性对照组给予生理盐水，辐射模型组单纯接受照射，3个五指毛桃剂量组分别给予相当于生药5，10，20 g·kg-1·d-1五指毛桃水提液，连续灌胃7 d。除阴性对照组外，所有小鼠接受全身一次性60Coγ射线照射，照射剂量为6 Gy。照射24 h后处死小鼠，取股骨，制作骨髓单细胞悬液，用单细胞凝胶电泳实验检测小鼠骨髓细胞DNA损伤情况。结果 辐射模型组小鼠骨髓细胞DNA的尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)高于阴性对照组(P<0.01)，五指毛桃各剂量组的Tail DNA%和Tail Moment均显著低于辐射模型组(P<0.01)，且随着给药剂量的增加，Tail DNA%和Tail Moment降低。结论 五指毛桃水提液对60Coγ射线损伤小鼠骨髓细胞DNA具有一定的防护作用。 |
| 关键词: 五指毛桃 60Coγ射线 骨髓细胞 DNA损伤 单细胞凝胶电泳 |
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| 基金项目:广西教育厅科研项目(200809MS124；200911MS195) |
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| Protective Role of Aqueous Extract from Fici Hirtae Radix for DNA Damage of Bone Marrow Cells by 60Coγ-Ray in Mice |
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WANG Xiaoping1,2, DUAN Liju3, HUANG Xiang1,2, CEN Yewen1,2, LI Guifen1,2
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1.Yulin Normal College, a.Department of Chemical and Biology;2.b.Department of Physical Education, Yulin 537000, China;3.College of Pubilc Heath, Zhengzhou University, Zhengzhou 450001, China
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| Abstract: |
| OBJECTIVE To investigate the protective role of aqueous extract from Fici Hirtae Radix for bone marrow cells DNA of 60Coγ-ray irradiated mice. METHODS Forty SPF male mice were randomized into 5 groups. Negative control group was given normal saline, irradiated group was radiation model group and 3 Fici Hirtae Radix dose groups were orally given aqueous extracts from Fici Hirtae Radix of 5, 10, 20 g·kg-1·d-1 for 7 consecutive days. Except the negative control group, all mice received 6 Gy irradiation of whole body. After 24 h of irradiation, mice were sacrificed on femur. Single cell suspension was produced. DNA damage was detected using single cell gel electrophoresis experiment. RESULTS The results showed that Tail DNA% and Tail Moment of radiation model mice were higher than negative control group (P<0.01). Tail DNA% and Tail Moment of all Fici Hirtae Radix dose groups were significantly lower than radiation model group(P<0.01). As the dose increased, Tail DNA% and Tail Moment reduced. CONCLUSION Aqueous extracts from Fici Hirtae Radix have good protective effect on 60Coγ-organization of bone marrow cells DNA damage in mice. |
| Key words: Fici Hirtae Radix 60Coγ-rays bone marrow cells DNA damage single-cell gel electrophoresis |
