| 引用本文: | 陈新阳,毕开顺,易丽昕,黄文芳,陈晓辉.双波长切换HPLC同时测定桂枝甘草汤中4种成分的含量[J].中国现代应用药学,2011,28(3):265-267. |
| CHEN Xinyang,BI Kaishun,YI Lixin,HUANG Wenfang,CHEN Xiaohui.Simultaneous Determination of Four Components in Guizhi-Gancao Decoction by HPLC with Double-wavelength Switch[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(3):265-267. |
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| 摘要: |
| 目的 建立桂枝甘草汤中4种成分同时含量测定的方法。方法 采用高效液相色谱法，Kromasil C18柱(4.6 mm×250 mm，5 mm)，以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱，流速：1.0 mL·min-1，双波长切换时间序列采样：0~29.5 min为276 nm；29.5~32.0 min为370 nm。结果 甘草苷、肉桂酸、桂皮醛、异甘草素分别在10.0~160，2.50~40.0，16.0~256，0.050~0.8 μg·mL-1内，线性关系良好。结论 该方法准确、简便、专属性好，可用于桂枝甘草汤中甘草苷、肉桂酸、桂皮醛和异甘草素4种成分的含量测定，为桂枝甘草汤的质量控制提供依据。 |
| 关键词: 高效液相色谱法 桂枝甘草汤 甘草苷 肉桂酸 桂皮醛 异甘草素 |
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| Simultaneous Determination of Four Components in Guizhi-Gancao Decoction by HPLC with Double-wavelength Switch |
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CHEN Xinyang, BI Kaishun, YI Lixin, HUANG Wenfang, CHEN Xiaohui
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School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
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| Abstract: |
| OBJECTIVE To develop an HPLC method for determination of liquiritin, cinnamic acid, cinnamaldehyde and isoliquiritigenin in Guizhi-Gancao decoction with gradient elution and double-wavelength switch. METHODS The analysis was carried out on a Kromasil C18(4.6 mm×250 mm, 5 μm) column. The mobile phase was composed of acetonitrile and 0.1% formic acid aqueous with gradient elution and the flow rate was set at 1.0 mL·min-1. The detection wavelengths were set at 276 nm for liquiritin, cinnamic acid and cinnamaldehyde (0-29.5 min) and 370 nm for isoliquiritigenin (29.5-32.0 min). The column temperature was 35 ℃. RESULTS The linear ranges of liquiritin, cinnamic acid, cinnamaldehyde and isoliquiritigenin were 10.0-160, 2.50-40.0, 16.0-256, 0.050-0.8 μg·mL-1, respectively. CONCLUSION This method can be used to determine the above 4 components with satisfied accuracy and repeatability. |
| Key words: HPLC Guizhi-Gancao decoction liquiritin cinnamic acid cinnamaldehyde isoliquiritigenin |
