| 引用本文: | 王昌明,黎洪秀,张孝飞.青蒿琥酯对人胚肺成纤维细胞Caspase-3表达的影响[J].中国现代应用药学,2011,28(1):11-14. |
| WANG Changming,LI Hongxiu,ZHANG Xiaofei.Effect of Artesunate on the Expression of Caspase-3 in Human Embryonic Lung Fibroblasts[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(1):11-14. |
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| 摘要: |
| 目的 研究青蒿琥酯对人胚肺成纤维细胞(human embryonic lung fibroblast, HELF)系HFL-I细胞体外生长的影响，为青蒿琥酯抗纤维化提供实验依据。方法 采用CCK-8法检测青蒿琥酯对体外培养的HFL-I细胞生长的影响，用流式细胞术测定细胞凋亡率；RT-PCR法测定凋亡相关蛋白Caspase-3 的mRNA表达水平，Western blot法分析Caspase-3蛋白的表达情况。结果 青蒿琥酯呈浓度依赖性抑制HFL-I细胞增殖，HFL-I细胞经青蒿琥酯作用后细胞主要停滞于G1期，凋亡率明显增加(P<0.01)，Caspase-3 mRNA的表达显著高于对照组，Caspase-3蛋白的表达亦显著高于对照组。结论 青蒿琥酯很可能通过上调Caspase-3 mRNA及蛋白的表达水平，进而抑制HFL-I细胞的增殖，并促进HFL-I细胞的凋亡，从而发挥抗肺纤维化作用。 |
| 关键词: 青蒿琥酯 人胚肺成纤维细胞 凋亡 Caspase-3 |
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| 基金项目:广西科学研究与技术开发计划项目(桂科攻10124008-4) |
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| Effect of Artesunate on the Expression of Caspase-3 in Human Embryonic Lung Fibroblasts |
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WANG Changming, LI Hongxiu, ZHANG Xiaofei
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The Affiliated Hospital of Guilin Medical College, Guilin 541001, China
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| Abstract: |
| To study the effect of artesunate on the growth of human embryonic lung fibroblast HFL-I cells in vitro and provide experimental data for anti-fibrosis activity of artesunate. METHODS CCK-8 assay was used to determine the effect of artesunate on the growth of HFL-I cells in vitro. Apoptosis ratio was examined by flow cytometry (FCM). The mRNA level of Caspase-3, one of apoptosis related proteins，were assessed by RT-PCR. The expression of Caspase-3 protein was detected by Western blot. RESULTS Artesunate has a significantly inhibitory effect on the proliferation of HFL-I cells in a dose-dependent manner in vitro. Flow cytometry assay demonstrated a higher distribution in G1 phase of HFL-I cells with artesunate. And apoptosis rate of HFL-I cells was significantly increased in artesunate-treated group compared with control group (P<0.01). RT-PCR analysis showed the levels of Caspase-3 mRNA were significantly increased in artesunate-treated group, compared with control group. Western bloting also showed a significant enhancement of Caspase-3 protein levels in artesunate-treated group, when compared to control group. CONCLUSION Artesunate may exert marked anti-pulmonary fibrosis effect by up-regulating the mRNA and protein level of Caspase-3, which could induce the growth inhibition and apoptosis in HFL-I cells. |
| Key words: artesunate human embryonic lung fibroblasts apoptosis Caspase-3 |
