| 引用本文: | 姜平川,李嘉,杨海船,张赟赟,李红,黄建猷.HPLC测定九里香中脱水长叶九里香内酯的含量[J].中国现代应用药学,2011,28(4):341-344. |
| JIANG Pingchuan,LI Jia,YANG Haichuan,ZHANG Yunyun,LI Hong,HUANG Jianyou.Determination of Phebalosin in Murrayae Folium et Cacumen by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(4):341-344. |
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| 摘要: |
| 目的 建立九里香药材中脱水长叶九里香内酯的含量测定方法。方法 采用高效液相色谱法，使用Agilent Esclipe XDB-C18色谱柱(150 mm×4.6 mm，5 μm)，流动相为甲醇-水(55∶45)，流速1 mL·min-1，检测波长332 nm，柱温25 ℃。 结果 脱水长叶九里香内酯进样浓度在20.72~207.2 μg·mL-1内，与峰面积呈良好的线性关系(r=0.999 8)；其平均回收率为98.41%，RSD为1.65%(n=6)。结论 本法简便易行，重复性好，可用于九里香药材中脱水长叶九里香内酯的含量测定。 |
| 关键词: 九里香 脱水长叶九里香内酯 高效液相色谱法 含量测定 |
| DOI： |
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| 基金项目:广西科技厅科技攻关项目(桂科攻0815005-1-22) |
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| Determination of Phebalosin in Murrayae Folium et Cacumen by HPLC |
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JIANG Pingchuan, LI Jia, YANG Haichuan, ZHANG Yunyun, LI Hong, HUANG Jianyou
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Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China
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| Abstract: |
| OBJECTIVE To establish a high performance liquid chromatographic method for the determination of phebalosin in Murrayae Folium et Cacumen. METHODS The analysis was carried out on an Agilent Esclipe XDB-C18 column(150 mm×4.6 mm, 5 μm), using a mixture of 55% methanol and 45% water as the mobile phase at a flow rate of 1 mL·min-1 at 25 ℃, the wavelength for measurement was 332 nm. RESULTS The calibration curve was linear in the range of 20.72-207.2 μg·mL-1(r=0.999 8); the average recovery was 98.41% with RSD of 1.65%(n=6). CONCLUSION The method is simple, accurate and reproducible, and it is suitable for the determination of phebalosin in Murrayae Folium et Cacumen. |
| Key words: Murrayae Folium et Cacumen phebalosin HPLC determination |
