| 引用本文: | 高剑坤,范开,侯再金,张兵兵,杨黎,胡春兰.重组人睫状神经营养因子脂质体制备及性质[J].中国现代应用药学,2011,28(2):146-149. |
| GAO Jiankun,FAN Kai,HOU Zaijin,ZHANG Bingbing,YANG Li,HU Chunlan.Preparation and Evaluation of Liposome-encapsulated Recombinant Human Ciliary Neurotrophic Factor[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(2):146-149. |
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| 摘要: |
| 目的 制备重组人睫状神经营养因子(recombinant human ciliary neurotrophic factor，rhCNTF)脂质体，提高rhCNTF穿透血脑屏障能力。方法 采用薄膜超声法制备rhCNTF脂质体，测定包封率。分别用rhCNTF原液、rhCNTF脂质体及生理盐水尾静脉注射Balb/C小鼠，0，2，6，12，24 h后测小鼠脑组织中rhCNTF含量。结果 制得的rhCNTF脂质体形态圆整，平均直径为85.0 nm，包封率为93.14%。用ELISA法测定小鼠脑组织中rhCNTF的含量，rhCNTF原液组和rhCNTF脂质体组存在显著差异。结论 制备的rhCNTF脂质体能提高rhCNTF穿越血脑屏障的能力，为rhCNTF的进一步应用和研究提供了依据。 |
| 关键词: 睫状神经营养因子 脂质体 血脑屏障 |
| DOI： |
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| 基金项目:绵阳市科技计划项目(08S002-4) |
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| Preparation and Evaluation of Liposome-encapsulated Recombinant Human Ciliary Neurotrophic Factor |
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GAO Jiankun1, FAN Kai2,3, HOU Zaijin1, ZHANG Bingbing4, YANG Li3, HU Chunlan3
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1.Sichuan College of Traditional Chinese Medicine, Mianyang 621000, China;2.Chongqing University of Technology, Chongqing 400050, China;3.Chongqing Fagen Biomedical Inc., Chongqing 400041, China;4.Bioengineering College of Chongqing University, Chongqing 400044, China
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| Abstract: |
| OBJECTIVE To enhance the penetration ability to cross the blood-brain barrier efficiently of recombinant human ciliary reurotrophic factor (rhCNTF) by encapsulated into liposome. METHODS rhCNTF liposomes were prepared by thin film dispersion-sonication method. Encapsulation efficiency was detected by HPLC. Mice were killed 0, 2, 6, 12 and 24 h after i.v. administration of rhCNTF solution, rhCNTF liposomes or saline, and then the content of rhCNTF in brain tissues was analyzed by ELISA. RESULTS rhCNTF liposomes were regular in its morphology with a mean diameter of 85.0 nm. The entrapment efficiency of rhCNTF was 93.14%. rhCNTF in mice brain tissue were different between rhCNTF solution group and rhCNTF liposomes group. CONCLUSION Liposome-encapsulated rhCNTF could efficiently cross the blood brain barrier, which provides a basis for further application and research of rhCNTF. |
| Key words: ciliary neurotrophic factor liposomes blood brain barrier |
