| 引用本文: | 薛培凤,陈玉荣,青梅,杨慧.HPLC测定蒙药曼陀罗子中东莨菪碱的含量[J].中国现代应用药学,2011,28(2):164-167. |
| XUE Peifeng,CHEN Yurong,QING Mei,YANG Hui.Determination of Scopolamine in Datura stramonium by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(2):164-167. |
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| 摘要: |
| 目的 建立曼陀罗子中东莨菪碱的含量测定方法。方法 色谱柱为Kromasil C18(250 mm×4.6 mm，5 mm)。乙腈-水(含0.07 mol·L-1磷酸钠和0.017 5 mol·L-1十二烷基硫酸钠，0.5%磷酸，0.1%三乙胺)(37∶63)为流动相，检测波长为216 nm。结果 东莨菪碱在0.038 1~0.190 5 mg·mL-1内呈良好的线性，r=0.999 9；平均回收率为97.0%，RSD为0.93%。结论 该方法简便、灵敏、准确、重复性好，可作为曼陀罗子的质量控制标准。 |
| 关键词: 曼陀罗子 东莨菪碱 含量测定 高效液相色谱法 |
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| 基金项目:内蒙古政府蒙药现代化重大研究专项蒙药材质量标准化资助项目(MX2005ZB015) |
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| Determination of Scopolamine in Datura stramonium by HPLC |
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XUE Peifeng, CHEN Yurong, QING Mei, YANG Hui
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School of Pharmacy, Inner Mongolia Medical College, Hohhot 010059, China
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| Abstract: |
| OBJECTIVE To establish the method of determination of scopolamine in Datura stramonium. METHODS Kromasil C18(250 mm×4.6 mm, 5 mm) column was employed. Mobile phase was the mixture of acetonitrile and Na3PO4 buffer (contains 0.07 mol·L-1 Na3PO4, 0.017 5 mol·L-1 SDS, 0.5% H3PO4, 0.1% trithylamine)(37∶63). RESULTS A good linearity of scopolamine was achieved in the range of 0.038 1-0.190 5 mg·mL-1(r=0.999 9). The average recovery of scopolamine was 96.97% and RSD was 0.93%. CONCLUSION This method is simple, sensitive, accurate with good reproducibility, and can be used for the quality control of Datura stramonium. |
| Key words: Datura stramonium scopolamine detetmination HPLC |
