| 摘要: |
| 目的 研究拳参乙醇提取物(BRE)的免疫调节作用。方法 在小鼠体外实验中，通过比色分析法检测小鼠巨噬细胞系RAW264.7细胞吞噬中性红的能力。在小鼠体内实验中，通过测定胸腺、脾脏重量并计算脏器指数；MTT法检测T淋巴细胞增殖和NK细胞活性；鸡红细胞免疫后测定小鼠血清溶血素抗体水平；ELISA法测定血清中IL-2含量。结果 BRE能够显著增强RAW264.7细胞的吞噬能力，增加正常小鼠胸腺和脾脏重量，促进T淋巴细胞增殖，增强NK细胞的细胞毒作用，上调血清溶血素水平及血清IL-2水平。结论 BRE具有一定的免疫调节作用。 |
| 关键词: 拳参 乙醇提取物 免疫调节 |
| DOI： |
| 分类号: |
| 基金项目:滨州医学院科技计划项目(BY2007KJ09) |
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| Immunoregulatory Function of Bistortae Rhizoma Ethanol Extract |
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LI Keke1, LUAN Xiying2
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1.Binzhou Medical University, College of Pharmacy, Yantai 264003, China;2.Binzhou Medical University, College of Basic Sciences, Yantai 264003, China
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| Abstract: |
| OBJECTIVE To study immunoregulatory function of Bistortae Rhizoma ethanol extract (BRE). METHODS The percentage of phagocytosis of neutral red by RAW264.7 cell line from mouse macrophages was detected by colorimetric assay, . normal mice, weigh the immunological organs, MTT method was employed to investigate T lymphocyte transformation efficiency and the cytotoxicity of NK cells; measurement of the level of serum hemolysin antibody in mice immunized by chicken red blood cells and the content of IL-2 in serum with ELISA. RESULTS BRE can improve phagocytosing function of RAW264.7 macrophage cell line, increase spleen index and thymus index, promote proliferation of T lymphocyte, increase the cytotoxicity of NK cells, raise the level of serum hemolysin antibody and the content of IL-2 in normal mice. CONCLUSION BRE possesses a potential immunoregulatory function. |
| Key words: Bistortae Rhizoma ethanol extract immunoregulatory |
