| 引用本文: | 李喜凤,郝哲,邱天宝,余云辉,杜云锋.蒲公英药材质量标准研究[J].中国现代应用药学,2011,28(1):54-57. |
| LI Xifeng,HAO Zhe,QIU Tianbao,YU Yunhui,DU Yunfeng.Studies on the Quality Standard of Hand.-Mazz[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(1):54-57. |
|
| 摘要: |
| 目的 对中药材蒲公英的水分、总灰分、酸不溶性灰分、浸出物及有效成分等物质质量进行研究，建立蒲公英药材的质量标准。方法 采用TLC对蒲公英中咖啡酸、绿原酸进行定性鉴别，应用HPCE对其有效成分进行含量测定，按中国药典2005年版一部附录Ⅸ H水分测定法中的烘干法、附录Ⅸ K灰分测定法、附录X A浸出物测定法测定。结果 咖啡酸、绿原酸分别在0.009 8~0.078 1 mg·mL-1，0.008 5~0.067 8 mg·mL-1内呈良好线性关系。蒲公英的水分含量不得超过10%，总灰分不得高于20%，酸不溶性灰分不得高于10%，热浸法水溶性浸出物量不低于25%，冷浸法水溶性浸出物量不低于20%。结论 此法简便、准确、能有效控制蒲公英药材的质量。 |
| 关键词: 蒲公英 质量标准 薄层色谱法 高效毛细管电泳法 |
| DOI： |
| 分类号: |
| 基金项目:河南省科技厅重点科技攻关资助项目(062306100) |
|
| Studies on the Quality Standard of Hand.-Mazz |
|
LI Xifeng1, HAO Zhe2, QIU Tianbao1, YU Yunhui1, DU Yunfeng1
|
|
1.Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China;2.The PLA 153 Central Hospital, Zhengzhou 450042, China
|
| Abstract: |
| OBJECTIVE To set up the quality standards of Hand.-Mazz through determination of the contents of water extracts, total ashes, ashes imdissolvable in acid, moisture and effective components in this herb. METHODS TLC was used for identification of caffeic acid and chlorogenic acid, and HPCE for content determination. These indexes were determined based on the procedures recorded in the Appendix IX H, IX K and X A of Ch.P(2005), for moisture, ashes and extracts determination respectively. RESULTS The calibration curve displayed good linear relationship within caffeic acid and chlorogenic acid, concentration range of 0.009 8-0.078 1 mg·mL-1, 0.008 5-0.067 8 mg·mL-1. The moisture in dandelion was less than 10%, with the total ashes content within 20%, ashes imdissolvable in acid not higher than 10%, the content of water extracts with hot soak was higher than 25%, the content of water extracts was higher than 20%. CONCLUSION These datas are quantitatively referable for making the quality standards of Hand.-Mazz better. |
| Key words: dandelion quality standard TLC HPCE |
